Anti-Diarrhoeal Activity and Toxicity Trial of Methanolic Fruit-Pulp Extract of Aegle Marmelos (L.) Correa in Sprague-Dawle Rats

Abstract

To determine the toxicity as well as anti-diarrhoeal effect of the unripe methanolic fruit-pulp extract of Aegle marmelos in SD rats. Qualitative Phytochemical analysis of methanolic un ripe fruit-pulp extract of Aegle marmelos was done as per standard method. Extracts of methanolic unripe fruit-pulp extract of Aegle marmelos were evaluated at doses of 15mg/kg, 30mg/kg, 120mg/kg and 1600mg/kg in SD rats for anti diarrhoeal effect in castor oil induced diarrhoea. For Toxicity effect, 5000 mg/kg of methanolic extract of Aegle marmelos was administered orally to three female rats at first with 0.02% tween 80 vehicles and observed for any abnormal condition up to 14days .Statistical analysis was conducted by ANOVA following post hoc test. Qualitative analyses of different phytochemicals of methanolic extract of Aegle marmelos showed that the presence of Tannin and Terpenoids were highly positive whereas Alkaloid was moderately positive and Flavonoid was slightly positive. The study revealed that the dose of 30mg/kg BW and 1600 mg/kg BW of methanolic extract of A. marmelos showed same treatment response with Loperamide against castor oil induced diarrhoea in rats. Safety study did not show any side effects of high dose of A. marmelos therapy during rat model study. It can be concluded that methanolic extract of Aegle marmelos may be effective in reducing diarrhoea in animals