Anti-Diabetic mechanism of colchicum speciosum hydroalcoholic extract in vitro

Abstract

Diabetes is a chronic metabolic disease, which has various treatments and recently Anti-Diabetic effects within herbal medicines and their mechanism have drawn the attention.This study was purposed to investigate the Anti-Diabetic effects of hydroalcoholic extracts of colchicum Speciosum in vitro conditions on three mechanisms for insulin release, glucose uptake and hemoglobin glycosylation. In preparing hydroalcoholic extract of the herb, soaking was used in concentrations 100,200,400,600,800 and 1000. The rate of releasing insulin from calf’s pancreas cells in presence and absence of extract was assayed by ELISA kit. The rate of glucose uptake was also measured on the plasma with red cells in the presence and absence of the extract during 3 hours, which was done in every 1 hour. The rate of hemoglobin glycosylation was assayed in the presence and absence of the extracts and the rate of glycosylation inhibition was calculated. The extracts of colchicum could decrease releasing insulin significantly (p-value ≤ 0.05). While the glucose uptake was raised significantly ( p-value ≤ 0.05) . Further, colchicum extract could enhance hemoglobin glycosylation inhibition. According the above results, colchicum Speciosum may have Anti-Diabetic properties and it can be used as an Anti-Diabetic medicine and as a nutritional supplement for diabetics. Surely, similar studies are necessary in this field.