ANTI-DIABETIC EFFECT OF Acacia catechu MEDIATED SELENIUM NANOPARTICLES

Abstract

Selenium nanoparticles have made their way into the field of biomedicine through their application as antioxidants, antidiabetic agents, anticarcinogenic agents, nutritional supplements, and pharmaceutical supplements. The objective of the current study is to assess any potential antidiabetic effects of selenium nanoparticles using Acacia catechu. The solution of the nanoparticles was centrifuged at 8000 rpm for 10 minutes, collected, twice rinsed with distilled water, dried at 600 ºC, and stored. Se NP concentrations of 100, 200, 300, and 400 L were preincubated at room temperature for 30 min with 400 L of an alpha-amylase solution (1 U/mL). Equal amounts of the enzyme and extract were swapped out for a sodium phosphate buffer that was kept at a pH of 6.9. At 540 nm, the reading was recorded. Three times the experiment was run. In order to calculate the percentage of inhibition, acarbose was utilized as a positive control. The findings of the present investigation showed that Se- NPs exhibited anti-diabetic effects that were comparable to those of the reference standard. At a concentration of 100 l, the alpha amylase enzyme was inhibited by 57.2%, which was noticeably equivalent to the usual acarbose employed. According to the results of the current study, selenium nanoparticles have an inhibitory effect on alpha amylase, which unintentionally treats diabetes mellitus by regulating blood glucose levels. They exhibit significant alpha-amylase inhibition in relation to acarbose. Se NPs could thus offer a potential replacement for traditional antidiabetic medications in the future. According to the research, the synthesized selenium nanoparticles were spherical and small. According to in vitro antidiabetic functions research, the particles exhibit increased levels of alpha-amylase and alpha-glucosidase inhibitory actions. Additionally, it was less cytotoxic and had stronger antioxidant activity. The greenly synthesised selenium nanoparticles have shown promise as a natural treatment for diabetic mellitus.