Abstract
Objective: The study was to optimize the method of test dosage form kabau seed simplicia suspension in alloxan-induced diabetic rat models.
 Methods: Simplisia powder sizing was carried out using three sieve sizes through meshes of 80, 120, and 200. Making a suspension of kabau seed powder is carried out using two methods, with the addition of CMC, which has been developed first and the powder is added with CMC, which is then developed and then homogenized. The study consisted of six groups, dosage given of 250, 500, and 1000 mg/kg BW. Blood glucose levels were determined by the GOD-PAP, phytochemical screening and TLC tests were carried out on the test material. The data were tested statistically using One Way ANOVA.
 Results: The powdered crude material with a particle size of 74 microns, obtained by sieving through a mesh size of 200, is utilized as the test material in the second method. An effective dose of 1000 mg/kg BW with a decrease in blood sugar levels by 132±13 mg/dl. Phytochemical screening contained alkaloids, flavonoids, phenolics, monoterpenes and sesquiterpenes, steroids, quinones and saponins. TLC of the test material, mobile phase used in TLC was toluene: chloroform: ethyl acetate (4:5:1), obtained 6 spots which gives a specific spot after being sprayed with the chromogenic reagent.
 Conclusion: powder with a particle size of 74 microns was used as the test material, using the second method. The effective dose as an antidiabetic is 1000 mg/kg BW with a reduction in blood sugar levels of 132.7±13 mg/dl.
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