Abstract
Background: The relationship between the treatment of depression and plant-derived substances (e.g., flavonoids, coumarin, and scopoletin) has been demonstrated through interference with the monoamine system. The present study was planned to evaluate the anti-depressant effects of Asperula odorata L. plant through behavioral tests in mice. Material and Methods: In this experimental study, 35 male Syrian mice weighing 30-40 g were examined in five groups (n=7) as follow: received oral distilled water gavage (control), 10 mg/kg of fluoxetine solution gavage (reference standard), 10, 5, and 2.5 mg/kg of A. odorata L. extract gavage (treatment groups). After one week, all behavioral tests, including tail suspension test (TST), forced swimming test (FST), open field test (OFT), elevated plus maze test (EPMT), and fractionation tests were performed each morning for 4-6 h within five days. Results: The hydroalcoholic extract of A. odorata contained phenolic and flavonoid substances (Shinoda test confirmed flavonoid family). Administration of extract (10 and 5 mg/kg doses) versus fluoxetine (10 mg/kg dose) reduced the immobility of animals in both FST and TST (P<0.05). At the OFT, the administered extract increased the number of central square entries of animals with higher mobility (P<0.05). At a 10 mg/kg dose, the active flavonoid ingredients increased the mice's incline to entre and spent more time within no wall parts of EPMT (P<0.05). Conclusion: Our study suggests that the hydroalcoholic extract of A. odorata L. could have significant anti-depressant activity. [GMJ.2021;10:e2206]
Highlights
Depression is one of the most common and recurrent psychological disorders worldwide
The mixture was passed through Whatman filter paper No 3 to obtain the extract resulting in 6.104 g of hydroalcoholic extract (HAE) from the 93g of dry powder, and the remainder of the powder was used for fractionation test to prove the presence of flavonoids, phenolics, and terpenoids
Fractionation The tests' results indicated that the HES of A. odorata L. contains some amounts of phenolics, terpenoids, and flavonoids
Summary
Depression is one of the most common and recurrent psychological disorders worldwide. Anti-depression Effect of Asperula odorata L usually caused by a combination of genetic, physical, environmental, and psychological factors. It is native to Europe, Northern America, Ukraine, and Russia [13] It has been demonstrated by 40 species in Ukraine, the most common one being A. orodata L. The anti-depressant properties of some of these species have been proved [14] These substances influence the genes of neurotransmitter receptors with antidepressant effects, such as noradrenaline (NE), serotonin (5-HT), dopamine (DA), and 5-Hydroxyindoleacetic acid (5-HIAA). The present study aimed to explore the antidepressant effects of A. orodata L. on the adult male laboratory mice using the behavior and fractionation tests. The present study was planned to evaluate the anti-depressant effects of Asperula odorata L. plant through behavioral tests in mice. Conclusion: Our study suggests that the hydroalcoholic extract of A. odorata L. could have significant anti-depressant activity
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