Anti-cytokine effects of chalcon analogues in experimental "cytokine storm" in rats

Abstract

The aim of the study was to evaluate the anti-cytokine effects of chalcon analogues in an experimental cytokine storm.
 Materials and methods. The cytokine storm was modeled in rats by intraperitoneal injection of lipopolysaccharide at a dose of 10 mg / kg. The test compounds and the reference drug dexamethasone were administered intraperitoneal 60 minutes after lipopolysaccharide injection at doses of 20 mg / kg and 3 mg / kg, respectively. After 24 hours, changes of the cytokines concentration in blood serum (IL-1, IL-6, IL-10, and TNF-), body temperature, and the severity of pulmonary edema were evaluated.
 Results. In the study, it was found that the administration of all the test-compounds reduced symptoms of hypercytokinemia, reflected in the decrease in the concentration of proinflammatory cytokines IL-1, IL-6, and TNF- with high content of IL-10 in serum. At the same time, the body temperature and pulmonary edema in rats against the background of the injection of the test chalcon analogues relative to animals that did not receive pharmacological support also decreased. Against the background of the administration of dexamethasone to animals, the concentration of IL-6, IL-1 and TNF- decreased by 25.0% (p 0.05); 44.1% (p 0.05) and 33.3% (p 0.05), as well as an increase in the content of IL-10 by 60.0% (p 0.05), with a decrease in pulmonary edema and body temperature. It should be noted that there were no statistically significant differences between the groups of animals that were received the studied chalcon analogues and the reference drug.
 Conclusion. The study showed the relevance of further study of representatives of a number of chalcon derivatives as non-hormonal means of correcting the cytokine storm with a high therapeutic potential.