Anti-carcinogenic effects of the phenolic-rich extract from abnormal Savda Munziq in association with its cytotoxicity, apoptosis-inducing properties and telomerase activity in human cervical cancer cells (SiHa).

Guzalnur Abliz,Fatima Mijit,Tangnur Ablimit,Li Hua,Nurmuhammat Amat,Guzalnur Abdixkur,Halmurat Upur

doi:10.1186/s12906-015-0530-x

Abstract

BackgroundAbnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment cancer. The polyphenol is main compounds contained in ASMq preparation responsible for anticancer effect of ASMq.MethodsIn this study,Real-time quantitative Polymerase Chain Reaction (RT-PCR) assay, MTT assay and flow cytometry were used to investigate the effect of polyphenol of ASMq on cell viability and the potential of the phenolic rich extracts of ASMq to induce apoptosis in human cervical cancer cells SiHa and its effects on telomerase activity were investigated. Cellular morphological change was observed by phase contrast microscopy. The MTT cell viability data revealed that treatment with phenolic rich extracts at 75 ~ 175 μg/ml significantly inhibited the viability and proliferation of cells, and these effects occurred in a concentration-dependent manner and time dependent manner (P < 0.01).ResultsThe phenolic rich extracts can induce apoptosis of SiHa cells, can increase the apoptosis rate in a concentration-dependent manner and time dependent manner (P < 0.01). Growth inhibition and apoptosis induction by phenolic rich extracts treatment on SiHa cells was associated with down-regulation of anti-apoptotic Bcl-2 expression and telomerase (P < 0.05) and Survivin expression. In addition, phenolic rich extracts exerted a dose-dependent induction of FHIT expression.ConclusionThese results suggest that phenolic rich extracts may have anti-tumor effects in human cervical cancer through cytotoxicity, apoptosis-inducing properties and telomerase activity.

Highlights

Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment cancer
Cytotoxicity and inhbiitory activity of ASMq extract on cell morphology and cell proliferation To evaluate the effects of ASMp on cell proliferation, we investigated the effects of ASMqp on cell growth of Siha cells
This provides a molecular basis for understanding the chemopreventive effect of ASMqp that might be ideal candidates to induce effective apoptosis in human cervical cancer cells

Summary

Introduction

Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment cancer. The worldwide incidence has decreased, primarily because of the widespread use of Cancer is a multifactorial and multistep disease caused by the accumulation of multiple hits which involves genetic and epigenetic alterations leading to aberrant expression of genes involved in initiation, progression and promotion of carcinogenesis [4]. Apoptosis is tightly regulated by a number of gene products that promote or block cell death at different stages. There are apparently many factors that have been demonstrated to be critical in the regulation of apoptosis, including Bcl-2 family, FHIT and Survivin, involved in the apoptotic process through the expression of genes, and the characterization of the function of these gene products will help to define the process of cell death at the molecular levels [7,8,9]

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