Anti-cancer Properties of Dietary Supplement CELNORM against Colon and Lung Cancer: An in vitro preliminary study

Abstract

Cancer is a complex disease characterized by a cascade of events that culminate in the accumulation of several genetic alterations. Because of the high incidence and mortality rate, scientists began seeking novel medications that were harmful to cancer cells but not to healthy cells. This study aimed to investigate the effect of CELNORM and its various components individually in colon and lung cancer cells. Based on the LD50 value, colon and lung cancer cells were treated with two different dosages of curcumin, pepper, carrot and cucumber extracts, individually and CELNORM (formulation with all four ingredients). The cytotoxicity of each compound has been checked through morphological analysis and cell viability assay by using the CCK8 kit and statistically analyzed. qPCR-based Gene expression study has also been done to further validate the anti-cancerous properties of the compounds. Curcumin extract decreased cancer cell growth, as evidenced by cytotoxicity and morphological analyses, but pepper, carrot, and cucumber extracts showed a less significant reduction in cancer cell growth. CELNORM, on the other hand, had the highest significant effect in suppressing cell proliferation, indicating the novel health supplement CELNORM renders more efficacy than individual components. Gene expression analysis also verifies the anti-cancerous properties of CELNORM, though some gene expressions are not in the expected line. The morphological analysis and cell viability result has shown the efficacy of the CELNORM over the individual compounds of the CELNORM and gene expression analysis of proliferative genes such as Cyclin D, CDK6, PCNA and BCL-2 further validates the anti-cancerous properties of CELNORM and all four components.