Abstract
Objectives To evaluate the anticancer properties of cephaeline in an oral squamous cell carcinoma (OSCC) cell line, including in cancer stem cells (CSC) regulation. Study Design The SCC-9 cell line was used as an in vitro OSCC model. Briefly, cell viability was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and cell migration by wound healing scratch assay. Gistone acetylation (H3K9ac) status was determined by immunofluorescence staining. CSC was evaluated by clonogenic assay. Results A single-dose administration of cephaeline reduced viability of OSCC cells and delayed cell migration. Moreover, cephaeline halted CSC colony formation. Additionally, cephaeline affected histone acetylation status. Conclusions Our findings suggest that cephaeline has anti-cancer properties in OSCC. Further analysis with other cell lines and in vivo studies are needed to validate these findings. To evaluate the anticancer properties of cephaeline in an oral squamous cell carcinoma (OSCC) cell line, including in cancer stem cells (CSC) regulation. The SCC-9 cell line was used as an in vitro OSCC model. Briefly, cell viability was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and cell migration by wound healing scratch assay. Gistone acetylation (H3K9ac) status was determined by immunofluorescence staining. CSC was evaluated by clonogenic assay. A single-dose administration of cephaeline reduced viability of OSCC cells and delayed cell migration. Moreover, cephaeline halted CSC colony formation. Additionally, cephaeline affected histone acetylation status. Our findings suggest that cephaeline has anti-cancer properties in OSCC. Further analysis with other cell lines and in vivo studies are needed to validate these findings.
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