Anti cancer potential of green synthesized silver nanoparticles

Abstract

At present cancer claims lives of millions of people worldwide annually. Therefore in past few years focus of researchers to develop cancer therapeutics from natural products increasing significantly. In the present paper we are reporting our studies on the synthesis of phytofunctionalized silver nanoparticles (AgNPs) and their anti cancer activity against the human breast cancer (MCF-7) and mammary cancer (EVSA-7) cell lines by using MTT assay. In past few years green synthesis of nanoparticles using plant extracts has gained wide interest owing to its advantages such as eco-friendliness, cost effective, less time consuming and simple. For the very first time, silver nanoparticles were successfully synthesized using Phaseolus vulgaris seeds extract. The synthesized nanoparticles were then characterized by Uv-Vis. Absorption spectroscopy and the characteristic surface Plasmon resonance peak was obtained at 450 nm. X-ray diffraction study was used to ascertain the crystalline nature and purity of the synthesized nanoparticles. The powder XRD pattern confirms cubic structure with the average particle size 15.75 nm. Fourier transform infrared spectroscopy (FTIR) was used to key out the specific functional groups responsible for the reduction of silver nitrate to form silver nanoparticles and the capping agent present in the seed extract. The antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus Aureus and Klebsiela pneumonia were studied which would find applications in the development of new antibacterial drugs. The synthesized nanoparticles were further examined for their anticancer and antiulcer activity. The green synthesized nanoparticles were found to be effective in cyto-toxic activity against the human breast cancer (MCF-7) and mammary cancer (EVSA-7) cell lines. The synthesized silver nanoparticles were showed very promising results for anti cancer Activity.