Abstract
This study was carried out to synthesize quercetin (Qt) embedded poly(lactic acid) (PLA) nanoparticles (PLA–Qt) and to evaluate anti-cancer efficacy of PLA–Qt by using human breast cancer cells. PLA–Qt were synthesized by using novel emulsified nanoprecipitation technique with varying dimension of 32±8 to 152±9nm of PLA–Qt with 62±3% (w/w) entrapment efficiency by varying the concentration of polymer, emulsifier, drug and preparation temperature. The dimension of PLA–Qt was measured through transmission electron microscopy indicating larger particle size at higher concentration of PLA. The release rate of Qt from PLA–Qt was found to be more sustained for larger particle dimension (152±9nm) as compared to smaller particle dimension (32±8nm). Interaction between Qt and PLA was verified through spectroscopic and calorimetric methods. Delayed diffusion and stronger interaction in PLA–Qt caused the sustained delivery of Qt from the polymer matrix. In vitro cytotoxicity study indicate the killing of ∼50% breast cancer cells in two days at 100μg/ml of drug concentration while the ∼40% destruction of cells require 5 days for PLA–Qt (46±6nm; 20mg/ml of PLA). Thus our results propose anticancer efficacy of PLA–Qt nanoparticles in terms of its sustained release kinetics revealing novel vehicle for the treatment of cancer.
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