Anti-breast carcinoma effects of green synthesized nickel nanoparticles from Alhagi ‎sparsifolia leaf aqueous extract: A pre-clinical trial study ‎

Abstract

IntroductionMany nanoparticles have pharmacological and biochemical properties, including antioxidant and anti-‎inflammatory properties, which appear to be involved in anticarcinogenic and antimutagenic activities. In the recent study, nickel nanoparticles were green-synthesized using the Alhagi sparsifolia leaf aqueous ‎extract. ‎Material and methodsThe synthesized Ni nanoparticles‎ were characterized by analytical techniques including SEM, UV-Vis., and FT-‎IR. The nanoparticles were formed in a spherical shape in the average size of 16.19 nm. ‎ResultsIn the antioxidant test, the IC50 of Ni nanoparticles‎ and BHT against DPPH free radicals were 316 and 231 ‎‎µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with Ni ‎nanoparticles‎ were assessed by MTT assay for 48h about the cytotoxicity and anti-human breast cancer ‎properties on normal (HUVEC) and breast cancer cell lines i.e. lobular carcinoma of breast (UACC-3133), ‎inflammatory carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453). The viability of ‎malignant breast cell line reduced dose-dependently in the presence of Ni nanoparticles‎. The IC50 of Ni ‎nanoparticles‎ were 477, 548, and 605 µg/mL against lobular carcinoma of breast (UACC-3133), inflammatory ‎carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453) cell lines, respectively. ‎ConclusionsAfter clinical study, nickel nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to ‎formulate a new chemotherapeutic drug or supplement to treat the several types of human breast cancer. ‎