Anti-Bacterial Screening and Optimization of Lipids for the Preparation of Nanoemulsions

Abstract

The present study aimed to screen and optimized the levels of lipids and surfactants that holds an innate antibacterial activity against gram positive and gram negative bacteria. Gram positive bacteria (S. aureus and B. subtilis) were found to be more susceptible to various explored excipients as compared to Gram-negative bacteria (E. coli, P. aeruginosa). The mean particle of Smix in absence of oleylamine decreased from 300.32 ±5.64 nm to 58.43 ± 0.34 nm when cremophor RH 40 concentration increased from 0.25% w/v to 0.75% w/v. Thereafter, from 1.0% w/v to 2.0% w/v, there was insignificant change in mean particle size (10.34 ± 0.22 to 11.24 ± 0.32). Similarly, mean particle of Smix in presence of oleylamine decreased from 358.34 ± 8.32 nm to 110.45 ± 2.23 nm when cremophor RH 40 concentration increased from 0.25% w/v to 0.75% w/v. Thereafter, from 1.0% w/v to 2.0% w/v, the mean particle ranged between 54.21 ± 1.34 to 59.32 ± 1.43, illustrating insignificant variations.