Abstract
Five compounds including three triterpenoids and two sesquiterpenes were isolated from Alimatis rhizoma. Their chemical structures were determined to be alisol B 23-acetate (1), alisol C 23-acetate (2), alisol B (3), alismol (4) and alismoxide (5) by various spectroscopic analysis, including 1H-NMR, 13C-NMR, HMBC and MS spectra. Compounds 1–5 were evaluated for their antibacterial potential against 6 strains of bacteria including three drug-resistant bacteria (one methicillin-resistant Staphylococcus aureus strain CCARM 3506, two quinolone-resistant Staphylococcus aureus strains CCARM 3505 and CCARM 3519), two G+ bacteria (Streptococcus mutans KCTC 3289 and Staphylococcus aureus KCTC 209) and one G− bacterium (Escherichia coli KCTC 1924). Compounds 1–5 showed strong antibacterial effect against S. mutans KCTC 3289, their MIC values were 2, 64, 16, 32 and 32 μg/mL, respectively. The antibacterial activity results of compounds 1–5 against these bacteria were reported for the first time. The results indicate that Alimatis rhizoma are potential sources of new antibacterial material.
Highlights
Since the advent of antibiotics, it has cured countless infected patients around the world, but with the passage of time and people’s abuse of antibiotics, it has led to the emergence of drug-resistant strains
We demonstrated the isolation and structural characterization of three protostane-type triterpenoids (1–3) and two guaiane-type sesquiterpenes (4, 5) from the methanolic extract of A. rhizoma
Three tetracyclic triterpenoids (1–3) and two sesquiterpenes (4, 5) were isolated from the methanolic extract of A. rhizoma. Their chemical structures were determined as alisol B 23-acetate (1), alisol C 23-acetate (2), alisol B (3), alismol (4), alismoxide (5) (Fig. 1) using spectroscopic methods including 1D, 2D NMR (HMBC, HMQC) and TOF-MS spectra
Summary
Since the advent of antibiotics, it has cured countless infected patients around the world, but with the passage of time and people’s abuse of antibiotics, it has led to the emergence of drug-resistant strains. If the trend of bacterial resistance is not effectively controlled, human will fall into the crisis of no drugs available, so it is urgent to separate active compounds with good antibacterial activity from natural medicines. The concentrated EtOAc extract (50.89 g) was applied to normal phase silica gel (SiO2) column chromatography (CC), fully eluted with a gradient of dichloromethane (CH2Cl2)-MeOH (1:0–0:1) to obtain 13 fractions (EA–EM).
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