Anti-Angiogenic Effect of Clerodendreme inerme Leaves Extract

Abstract

Clerodendrum inerme, a medicinal plant, exhibits diverse pharmacological properties. Various species within the Clerodendrum genus have been traditionally utilized in indigenous medical systems and folk remedies. This study intended to scrutinize the anti-angiogenic possibility of CI through in-ovo, in-vitro, and in-vivo approaches. The screening methods employed include the chick chorioallantoic membrane test (in-ovo), sponge bud technique (in-vivo), as well as in-vitro assays for cell migration, cell proliferation, along with morphogenesis. Statistical analyses involved Dunnett’s test for mean comparisons, utilizing one-way ANOVA. In the chick chorioallantoic membrane assay, both angiogenic score and branching points were evaluated. Notably, CI exhibited noteworthy antiangiogenic effects. In the sponge implantation technique, CI at specified dosages resulted in a substantial reduction in sponge weights, the formation of new vessels, and hemoglobin levels. The antiangiogenic effects were particularly pronounced with increasing doses. Furthermore, on endothelial cells, C. inerme demonstrated a dose-dependent decrease in migration, proliferation, and tube length, indicating potent antiangiogenic properties. In summary, this research underscores the considerable antiangiogenic potential of CI by various assays.