Anti-angiogenic Activity of Rosa canina Extracts, an Ex-vivo and In-vivo Study

Abstract

Angiogenesis, known as blood vessel growth from preexisting vasculature, occurs when proangiogenic overcomes the angiogenic factor. The most important angiogenic factors (promotors) of angiogenesis were vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (FGF), platelet derived growth factor (PDGF), angiogenin and tumor necrosis factor (TNFα). Rosa canina, commonly known as rose hip or dog rose, widely distributed in Europe, Asia and North America, belongs to the Rosacea family. Different biological compounds were found in R. canina, such as high phenolic composition (specifically flavonoids), vitamins as vitamin C, carotenoids, and fatty acids as (linolenic acid). Polyphenolic compounds are very important due to its efficacy as antioxidants and antiangiogenic. The dried powder of R. canina was extracted by using successive solvent extraction according to polarity (hexane, ethyl acetate and ethanol). The yield% of hexane, ethyl acetate and ethanol extracts were (0.8, 1.2 and 2.4 g per 100 g dried powder). Rat aorta assay (ex-vivo) was done to investigate antiangiogenic activity and choose the most bioactive extract. IC50 of avastin (positive control), ethanol, ethyl acetate and hexane were 20.281, 33.582, 61.744 and 94.537, respectively. The results revealed that ethanol extract (EE) was the most biologically active extract. Chorioallantoic membrane assay (CAM) in-vivo was done using R. canina ethanol extract. eosinophilic esophagitis (EE) has a greater percentage of inhibition of blood vessels when used in two concentrations 250 and 500 mg/20 mL. The zone of inhibition as mean ± SD was 19.921 ± 4.048 mm and 30.302 ± 2.805 mm, respectively. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), was used to identify the phytochemical constituents of the EE. In the present study, EE of R. canina revealed six flavonoid compounds (rutin, quercetin, catechine, astragalin, hyperoside and gallic acid) that could be responsible for the biological activity of the ethanol extract. In conclusion, the EE of R. canina reported a promising antiangiogenic activity both ex-vivo and in-vivo that may be attributed to the flavonoid content of the extract. The purpose from the study in order to investigate the antiangiogenic activity of R. canina extracts ex-vivo and in-vivo.