Abstract
ObjectiveTo investigate the effects and possible mechanism of Huangzhi Neiyi capsule (HZNY) on angiogenesis in rats with endometriosis.MethodsSixty Sprague-Dawley rats were used to establish an endometriosis model by autologous transplantation and were randomly divided into sham operation, model, gestrinone (0.25 g/kg), and HZNY (4.5 and 9 g/kg) groups. All drugs were administered orally for 28 days. At the end of the experiment, all rats were killed by cervical dislocation, and the expression of proliferating cell nuclear antigen (PCNA) and CD31 in ectopic endometrial tissue was assessed by immunohistochemistry. The level of vascular endothelial growth factor (VEGF) in peritoneal fluid was determined by enzyme-linked immunosorbent assay. The mRNA expression of VEGF and hypoxia inducible factor-1α (HIF1A) in ectopic endometrium was evaluated by reverse transcription-PCR.ResultsHZNY suppressed the expression of PCNA and CD31 in ectopic endometrium, reduced concentrations of VEGF in peritoneal fluid, and reduced mRNA expression of VEGF and HIF1A in ectopic endometrium.ConclusionsHZNY suppressed angiogenesis in endometriosis rats, perhaps by inhibiting expression of VEGF and HIF1A.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
