Anti- Alzheimer’s and Anti- Oxidant Activity of Cyamopsis tetragonoloba Fruit Extract in Mice

Abstract

The present study was designed to investigate the anti-alzheimer’s activity of Cyamopsis tetragonoloba fruit in mice. The two exteroceptive models, scopolamine (1mg/kg) and diazepam (1mg/kg) induced amnesia were used to study the anti-alzheimer’s effect of ethanolic fruit extract of Cyamopsis tetragoloba (CTFE) at the oral dose of 200mg/kg and 400mg/kg in Wistar albino mice. Piracetam (400mg/kg) was used as a standard drug. After completion of the 10 days respective treatments, scopolamine induced amnesia model showed the better activity in all the three; Elevated plus maze, Radial arm maze and Y maze parameters. The CFE at a dose of 200mg/kg and 400mg/kg, improved memory in a dose dependent manner. In scopolamine and diazepam induced amnesia model, biochemical parameters like lipid hydroperoxide (P<0.05) and malondialdehyde (P<0.01) levels were significantly decreased with administration of CFE (400mg/kg) when compared to scopolamine control. Whereas, acetylcholinesterase (P<0.01) level was significantly increased when compared to scopolamine control on administration of CFE (400mg/kg). In-vivo antioxidants like superoxide dismutase, reduced glutathione and catalase were decreased on inducing amnesia by scopolamine and diazepam when compared to normal group. Treatment with 400mg/kg of CFE significantly increased the levels of superoxide dismutase (P<0.05), reduced glutathione (P<0.05) and catalase (P<0.05) when compared to negative controls. From the results it was concluded that the ethanolic fruit extract of Cyamopsis tetragonoloba elicits anti-alzheimer’s activity in experimental animal models.