Anti-allodynic effect induced by curcumin in neuropathic rat is mediated through the NO-cyclic-GMP-ATP sensitive K+ channels pathway

Tracy Pastrana-Quintos,Julia Pérez-Ramos,Beatriz Godínez-Chaparro,Arrigo Coen,Giovanna Salgado-Moreno

doi:10.1186/s12906-020-2867-z

Abstract

BackgroundRecent studies pointed up that curcumin produces an anti-nociceptive effect in inflammatory and neuropathic pain. However, the possible mechanisms of action that underline the anti-allodynic effect induced by curcumin are not yet established. The purpose of this study was to determine the possible anti-allodynic effect of curcumin in rats with L5-L6 spinal nerve ligation (SNL). Furthermore, we study the possible participation of the NO-cyclic GMP-ATP-sensitive K+ channels pathway in the anti-allodynic effect induced by curcumin.MethodsTactile allodynia was measured using von Frey filaments by the up-down method in female Wistar rats subjected to SNL model of neuropathic pain.ResultsIntrathecal and oral administration of curcumin prevented, in a dose-dependent fashion, SNL-induced tactile allodynia. The anti-allodynic effect induced by curcumin was prevented by the intrathecal administration of L-NAME (100 μg/rat, a non-selective nitric oxide synthase inhibitor), ODQ (10 μg/rat, an inhibitor of guanylate-cyclase), and glibenclamide (50 μg/rat, channel blocker of ATP-sensitive K+ channels).ConclusionsThese data suggest that the anti-allodynic effect induced by curcumin is mediated, at least in part, by the NO-cyclic GMP-ATP-sensitive K+ channels pathway in the SNL model of neuropathic pain in rats.

Highlights

Recent studies pointed up that curcumin produces an anti-nociceptive effect in inflammatory and neuropathic pain
Curcumin reversed the development of mechanical allodynia suppressing the activation of Extracellular-signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in the spinal dorsal root ganglion (DRG) [15]
14 days after the ligation of the Lumbar 5 – Lumbar 6 (L5-L6) spinal nerves (SNL), the basal values of the 50% paw withdrawal threshold was disminished (≤ 4 g) in the ipsilateral paw as compared to sham group (15 g), indicating that the ligation of the L5-L6 spinal nerves induced tactile allodynia

Summary

Introduction

Recent studies pointed up that curcumin produces an anti-nociceptive effect in inflammatory and neuropathic pain. The purpose of this study was to determine the possible anti-allodynic effect of curcumin in rats with L5-L6 spinal nerve ligation (SNL). We study the possible participation of the NOcyclic GMP-ATP-sensitive K+ channels pathway in the anti-allodynic effect induced by curcumin. Previous studies have indicated that natural products produce anti-nociceptive and anti-allodynic effects through the NO-cGMP-ATP sensitive channels K+ pathway [20,21,22,23,24,25,26]. This work was undertaken to determine the possible anti-allodynic effect of curcumin in rats with spinal nerve ligation (SNL) model of neuropathy. We investigated whether, at the central level, the NO-cGMP-ATP sensitive channels K+ pathway participates in the antiallodynic effect induced by curcumin

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