Abstract
BackgroundBiyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.MethodsThe anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.ResultsEBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.ConclusionsTaken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.
Highlights
Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR)
Effect of extract of Biyeom-Tang (EBT) on β-Hex release and eicosanoid (PGD2 and leukotriene C4 (LTC4)) production The effects of EBT concentration (50, 100, and 200 μg/ml) on cell viability were assessed by an MTS assay and there was no significant change in cell viability observed in the response to these concentrations (Figure 2A)
Degranulation was monitored by determining the release of β-Hex because histamine release by activated mast cells parallels the release of β-Hex
Summary
Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). Allergic rhinitis (AR) is a common chronic disease of nasal mucosa characterized by IgE-mediated allergic inflammation [1]. It is not life-threatening, AR can deteriorate the quality of life because of the clinical symptoms including sneezing, rhinorrhea, itching and nasal congestion. These symptoms are primarily induced by allergic mediators released from mast cells, basophils, eosinophils, lymphocytes, and epithelial cells. Attention has focused on avoiding the adverse effects of medicines traditionally used to treat AR, which has stimulated the search for effective and safe alternatives
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