Abstract
Purpose: To determine the anti-aging activity and toxicity doses of phytooxyresveratrol extracted from Artocarpus lakoocha Roxb. Methods: Artocarpus lakoocha 100 g was extracted with 2 ml of 95 % ethanol to obtain phytooxyresveratrol (POV). Total phenolic content, as well as free radical scavenging and anti-glycation activities of POV were characterized in order to assess its anti-aging properties. The models of DNA nicking and bacterial reverse mutation (Ames) were applied to the extract in order to determine its effective and toxic doses, respectively.Results: Phytooxyresveratrol (POV) exhibited antiaging activity. It also showed high levels of phenolic content, radical scavenging activity and anti-glycation. A POV concentration of 25 μg/ml promoted strong anti-DNA nicking. Furthermore, it was non-toxic at concentrations ranging from 5 - 100 μg/ml.Conclusion: In vitro, phytooxyresveratrol (POV) extracted from Artocarpus lakoocha exerted effective anti-aging activity at a concentration of 25 μg/ml. Thus, the substance should be further studied for possible formulation into pharmaceutical products.Keywords: Aging, i, Free radicals, Glycation, Phytooxyresveratrol, DNA nicking.
Highlights
Oxidative damage to nucleic acid, lipid membrane and protein are associated with progressive loss of cellular structure and function [1]
We investigated the effective anti-aging dose of phytooxyresveratrol (POV) isolated from Artocarpus lakoocha
Phytooxyresveratrol was isolated by a high performance liquid chromatography (HPLC) and its structure confirmed with the 1H and 13C-NMR spectroscopic methods
Summary
Oxidative damage to nucleic acid, lipid membrane and protein are associated with progressive loss of cellular structure and function [1]. Artocarpus lakoocha is a plant used in traditional Thai medicine for anti-inflammatory therapy and as well as an anti-skin aging agent [8]. We investigated the effective anti-aging dose of phytooxyresveratrol (POV) isolated from Artocarpus lakoocha. To this end, total phenolic content, free radical scavenging and antiglycation were defined for anti-aging activity. The dried extract was obtained by drying the supernatant in a rotary evaporator at 50 oC and 50 mmHg. Phytooxyresveratrol was isolated by a high performance liquid chromatography (HPLC) and its structure confirmed with the 1H and 13C-NMR spectroscopic methods. Phenolic content of the sample was expressed as mg gallic acid/g sample equivalent based on the calibration standard curve of gallic acid standard
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