Abstract
BackgroundBlood stasis syndrome (BSS) is a general pattern identification and refers to pathological stagnation of blood circulation, dysfunction of endothelial cells or metabolic disorder in traditional Korean medicine (TKM). Dohongsamul-Tang (DHSMT) is a well-known traditional herbal formula which used for treatment and prevention of BSS by promoting blood circulation in TKM.MethodsCytotoxicity of DHSMT was examined by cell counting kit-8 (CCK-8). We also investigated the anti-adipogenesis effect of DHSMT by using Oil Red O staining, intracellular triglyceride assay leptin ELISA and western blot analysis in 3T3-L1 adipocytes. In addition, the accumulation of adiponectin, resistin and plasminogen activator inhibitor-1 (PAI-1) were measured by magnetic bead panel kit.ResultsOil Red O staining showed that DHSMT markedly reduced fat accumulation without affecting cell cytotoxicity. DHSMT also significantly decreased accumulation of triglyceride and adipokines such as leptin, adiponectin, resistin and PAI-1 compared with fully differentiated adipocytes. Furthermore, our results found that DHSMT significantly suppressed the adipocyte differentiation by downregulating adipogenic-specific transcriptional factors such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding proteins alpha (C/EBPα) and fatty acid binding protein 4 (FABP4) in adipocytes.ConclusionsTaken together, our findings provide that DHSMT has potential for treatment and prevention of obesity or MS related to BSS.
Highlights
Blood stasis syndrome (BSS) is a general pattern identification and refers to pathological stagnation of blood circulation, dysfunction of endothelial cells or metabolic disorder in traditional Korean medicine (TKM)
We evaluated the possible cytotoxicity of DHSMT in 3T3-L1 cells by using the cell counting kit-8 (CCK-8) assay
Our results revealed that DHSMT considerably down-regulated the protein expression of Proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding proteins alpha (C/EBPα) and fatty acid binding protein 4 (FABP4), which are essential for adipocyte differentiation and adipogenesis
Summary
Blood stasis syndrome (BSS) is a general pattern identification and refers to pathological stagnation of blood circulation, dysfunction of endothelial cells or metabolic disorder in traditional Korean medicine (TKM). Dohongsamul-Tang (DHSMT) is a well-known traditional herbal formula which used for treatment and prevention of BSS by promoting blood circulation in TKM. Blood stasis syndrome (BSS) is an important pathological concept in traditional Korean medicine (TKM) that was first recorded in Huangdi’s Inner. Several studies have reported that BSS is related to MS and its risk factors such as obesity, atherosclerosis, hypertension and diabetes mellitus [6,7,8]. Several herbal formulas such as Dohongsamul-Tang (DHSMT), Doinseunggi-Tang, Sobokchukeo-Tang, Hyeolbuchukeo-Tang have been widely used for treating BSS by circulating blood flow in TKM. Several studies have reported the effects of DHSMT, which include an anti-trombotic effect [10], an anti-inflammatory effect
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
