Anti‐adipogenic and anti‐inflammatory mechanism of nobiletin in co‐culture of adipocyte and macrophage (1045.13)

Abstract

Adipose tissues are characterized by the enhanced infiltration of macrophages. It is considered that the paracrine loop between adipocytes and macrophages establishes a vicious cycle that aggravates inflammatory changes and insulin resistance in obese adipose tissues. Nobiletin is abundantly present in the fruit peel of Citrus and has several health beneficial effects including antioxidant, anti‐inflammation and anticancer activities. However, not much is known about the anti‐adipogenic and anti‐inflammatory effect of nobiletin in co‐culture system of adipocyte and macrophage. The objective of this study was to evaluate the inhibitory effect of nobiletin on the interaction between adipocytes and macrophages. The results revealed that nobiletin markedly inhibited NO, TNF‐α and MCP‐1 secretion in contact and trans‐well systems of adipocytes and macrophages in a dose‐dependent manner. In addition, nobiletin blocked the expression of adipogenic transcription factors, including PPARγ, C/EBPα and aP2 in 3T3‐L1 cells of transwell system. Also, nobiletin inhibited the expression of iNOS in RAW264.7 cells. These results indicate that nobiletin exhibits anti‐adipogenic and anti‐inflammatory properties in the interaction of adipocytes and macrophages. Nobiletin may be useful for ameliorating the adipogenic and inflammatory changes in obese adipose tissues.