Abstract
Angiopteris helferiana C.Presl is a gigantic fleshy-type fern, belonging to Marattiaceae family. In previous study, we reported the potent anti-adipogenic and anti-inflammatory activities of ethylacetate (EtOAc) and n-butanol (BuOH) fractions of methanol extract of rhizomes of A. helferiana. In continuation, in this study, we report the isolation, characterization, and bioactivity analysis of principle bioactive compounds in these fractions. (−)-epi-Osmundalactone (1) and angiopteroside (2) were isolated from EtOAc and BuOH fractions, respectively. The structures of these compounds were established on the basis of NMR spectroscopic data. The quantification study using UPLC revealed the contents of compounds 1 and 2 in the dried rhizome to be 1.54% and 3.2%, respectively. These compounds were evaluated for their anti-adipogenic and anti-inflammatory activities using 3T3-L1 and RAW 264.7 cells, respectively. Compound 1 (2.5 µg/mL) and 2 (20 µg/mL) inhibited the lipid production by 35% and 25%, respectively. Regarding the anti-inflammatory activity, compound 1 (5 µg/mL) inhibited the nitrite production by nearly 82%. In conclusion, the presence of potent anti-adipogenic and anti-inflammatory compounds in A. helferiana indicate its potential role in the use of herb-based treatment for obesity and other related diseases.
Highlights
With the increasing modernization and lifestyle changes, metabolic disorder and related diseases are daily increasing and affecting a very large proportion of the global population
The presence of potent anti-adipogenic and anti-inflammatory compounds in A. helferiana indicate its potential role in the use of herb-based treatment for obesity and other related diseases
We reported that the ethylacetate (EtOAc) and n-butanol (BuOH) fractions of methanol extract of rhizomes of A. helferiana showed potent anti-adipogenic and anti-inflammatory activities [27]
Summary
With the increasing modernization and lifestyle changes, metabolic disorder and related diseases are daily increasing and affecting a very large proportion of the global population. Obesity is one specific example and rapidly growing public health problem [1]. The world health organization (WHO) has announced that obesity has reached the epidemic proportion worldwide [3]. Obesity is mainly associated with the increase in number and size of adipogenic cells called “adipocytes” which store large amount of fat and lipids [4]. It is an important risk factor for diabetes, hypertension, and cardiovascular diseases along with some chronic diseases such as stroke, osteoarthritis, sleep apnea, some cancers, and inflammation-bases pathologies [5,6,7].
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