Anthocyanins from Ipomoea batatas L. effect on ovarian malondialdehyde and 17β-estradiol in rats exposed cigarette smoke

Abstract

Background — Cigarette smoking negatively impacts the health of both active smokers and passive smokers. ROS (Reactive Oxygen Species) from cigarette smoke can cause damage to the ovary and inhibit ovarian follicle growth. Objectives — This study aimed to assess the effects of the anthocyanins from the sweet potato (Ipomoea batatas L.) purple cultivar on ovarian malondialdehyde (MDA) and 17β-estradiol levels in rats (Rattus norvegicus) exposed to cigarette smoke. Material and Methods — The Rattus norvegicus Wistar strain was used in this study. Thirty rats were divided into five groups. Cigarette smoke was given as two cigarettes/day for four minutes, in the morning and afternoon for eight weeks after rats were at the proestrus stage. MDA levelwere measured with the BIOXYTECH MDA-586TM spectrophotometric assay for malondialdehyde, whilst 17β-estradiol was measured by ELISA. Data were then analyzed using one-way ANOVA. Results — MDA level were significantly different between the negative control and positive control groups based on double comparison with the LSD test (p=0.000 <∝). The level of 17β-estradiol also showed asignificant difference between the negative and positive control groups. There was a significant correlation between MDA level and 17β-estradiol levels (p=0.000 <∝) in the group of rats exposed to cigarette smoke and treated with anthocyanins from sweet potato at doses of 40 mg and 80 mg. Conclusions — Anthocyanins from the sweet potato (Ipomoea batatas L.) purple cultivar given at various doses decreased ovarian MDA level and increased 17β-estradiol levels in female rats exposed to cigarette smoke.