ANTHOCYANIN PROFILING OF THE BERRY SKINS OF FIVE VITIS AMURENSIS GRAPES AND ONE RELATED HYBRID CULTIVAR

Abstract

V. amurensis is the world?s most northerly grapevine species and originates in the exceptionally cold climate in northeast China. Many V. amurensis grapes have been widely used because of not only their strong cold tolerance and disease resistance, but also high pigment content. However, there has been no detailed description of the anthocyanin composition and content in V. amurensis grapes. In this study, anthocyanin profiles in the berry skins of five V. amurensis grapes and one hybrid cultivar were analyzed by High Performance Liquid Chromatography-electronic Spray Ionization-tandem Mass (HPLC-ESI-MS/MS). The result showed that both the V. amurensis grapes and the hybrid cultivar contained 3,5-diglucosides of anthocyanidins in high amounts, in addition to 3-monoglucosides of delphinidin, cyanidin, petunidin, peonidin and malvidin that are generally detected in V. vinifera grapes. Furthermore, pelargonidin-3,5-diglucoside and B-type vitisin of certain anthocyanidin-3-glucoside were also identified in trace amount and no acetylated anthocyanins were detected in these samples. These findings will be important for the utilization of this germplasm in grapevine breeding programs and to further uncover biosynthesis and regulation of anthocyanins in V. amurensis grapes.