Anthocyanin Content of Fruit, Stem, Tendril, Leaf, and Leaf Petioles in Muscadine Grape

Abstract

Abstract Fruit, stems, tendrils, leaves, and leaf petioles of4Noble’ muscadine grape (Vitis rotundifolia Michx.) were analyzed using high performance liquid chromatography (HPLC) techniques. All five of the known 3,5-diglucoside forms of delphinidin (Dp), cyanidin (Cy), petunidin (Pt), peonidin (Pn), and malvidin (Mv) present in fruit of muscadine grape were detected in all sampled tissues except leaves, which contained only Dp, Cy, and Pt in detectable quantities. A sixth unknown pigment was detected in the fruit, and Dp 3-monoglucoside was detected in the leaves. Correlations were calculated to explore pigment relationships between fruit and vegetative tissues. Use of tendrils was best for predicting fruit Cy (r = 0.60), Mv (r = 0.57), Pn (r = 0.66), and Pt (r = 0.87). Use of stem tissue was best for predicting fruit Dp (r = 0.66). Prediction equations are given, and prediction of Cy could be improved by using both tendril and leaf measurements in a multiple regression (r = 0.80).