Abstract
The two major pigments of light-grown mustard ( Sinapis alba) seedlings were identified as cyanidin 3-sambubioside 5-glucoside and cyanidin 3-sambubioside 5-sophoroside esterified with malonic, p-coumaric, ferulic, and sinapic acids. The activities of the enzymes (2 S)-flavanone 3-hydroxylase and (+) dihydroflavanol 4-reductase and anthocyanin accumulation were determined in mustard seedlings at various times after illumination with red light (λ max = 660 nm). Both enzymes showed a drastic increase in activity after illumination and reached their maxima at the time of rapid anthocyanin accumulation. The results are consistent with the involvement of these enzymes in anthocyanin biosynthesis.
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