Abstract
Sampling procedures for determining anther length in wheat (Triticum aestivum L. em Thell.) were evaluated using variance components to calculate the relative efficiency of different anther sampling combinations of anthers/ floret, florets/spikelet, spikelets/spike, spikes/replication, and number of replications for two cultivars.Anther length is a useful indicator of anther size and is correlated with pollen grains per anther, a possible index of pollen available for cross‐pollination in hybrid wheat seed production.Variation for anther length among anthers within florets, florets within spikelets, and spikelets within spikes was small relative to variation among spikes and replications. Relative efficiency calculations indicated that a cultivar's anther length could be determined satisfactorily by measuring as few as one to three anthers/spike —with sufficient replication and sampling of spikes/plot. The relationship between relative efficiency and number of spikes sampled/plot was curvilinear; relative efficiency was improved only slightly by sampling more than 10 spikes/plot. Relative efficiency increased linearily with each additional replication for all sampling combinations.
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