Anther Culture for Double Haploid Breeding in Rice-a Way Forward

Abstract

Anther culture is an important biotechnological tool for quick recovery of fixed breeding lines in rice. It is largely species and genotype-specific. O. glaberrima responds better to anther culture than O. sativa; and japonica sub-group is more responsive to microspore embryogenesis than indica types. Media recipes and culture techniques have been standardized for callus induction and green plantlet regeneration using indica and japonica varieties as well as their inter se hybrids. Besides, the genetic basis and scope of anther culture for double haploid breeding in rice have been discussed.