Anterior pituitary gland secretion after forebrain ablation: periovulatory gonadotropin release.

Abstract

We sought to determine whether the estrous phase of FSH release in cyclic female rats is dependent on the immediate presence of the diencephalon. A piece of forebrain, the diencephalon and part of the telencephalon, was surgically removed from female rats between 1130--1300 or 2000-2200 h on proestrus. Blood was withdrawn through indwelling venous cannulae during the afternoon and evening of proestrus and the early morning of estrus for RIA of plasma LH, FSH, and PRL concentrations. In rats sham operated at either time period, plasma LH, FSH, and PRL levels rose from 1345 to 1800 h on proestrus. Whereas the plasma LH and PRL concentrations fell from 1800 h on proestrus to 0300 h on estrus, the plasma FSH concentrations remained elevated during this period. The removal of the piece of forebrain around noon on proestrus blocked the rises in the plasma LH and FSH levels and caused high plasma PRL concentrations from 1345 h on proestrus to 0300 h on estrus. The removal of the piece of forebrain during the evening of proestrus did not interfere with the fall in plasma LH concentrations or the maintenance of elevated plasma FSH concentrations during either late proestrus or the early morning estrus, but did cause high plasma PRL levels during that time interval. Hypophysectomy combined with removal of the forebrain piece during the evening of proestrus resulted in a drop in plasma FSH and PRL concentrations. The results confirm that in the rat, 1) the prosencephalon plays an acute stimulatory role n causing the preovulatory LH surge and the proestrous phase of FSH release, 2) the prosencephalon exerts effects during the afternoon and/or early evening of proestrus that cause the estrous phase of FSH release, and 3) the estrous phase of FSH release occurs in the absence of acute diencephalic stimulation.