Antennal Morphology and Fine Structure of Flagellar Sensilla in Hippoboscid Flies with Special Reference to Lipoptena fortisetosa (Diptera: Hippoboscidae).

Abstract

Simple SummaryIn insects, host searching usually involves different kinds of stimuli, both visual and chemical, that may act in combination. External cues are perceived through specific sensory organs (sensilla), mainly present on the antennae. Understanding how ectoparasites belonging to the Hippoboscidae locate their hosts is crucial, since these flies infest animals and can attack humans, with veterinary and medical implications. The aim of this research was to study the antennae of four hippoboscid species, Lipoptena cervi (Linnaeus, 1758), Lipoptena fortisetosa Maa, 1965, Hippobosca equina Linnaeus, 1758, and Pseudolynchia canariensis (Macquart, 1840), investigating the morphology and the sensory structures present on these appendages. A typical conformation of the antennae with the envelopment of the third segment (flagellum) inside the first two have been observed. Moreover, two types of sensilla have been detected and their role in the perception of host odours and CO2 have been hypothesized. Other antennal structures seem to be involved in the detection of temperature and humidity variations. Our findings confirm that these hippoboscids use chemoreception for host location, giving insights into this complex process in this poorly investigated group.Lipoptena cervi (Linnaeus), Lipoptena fortisetosa Maa, Hippobosca equina Linnaeus, and Pseudolynchia canariensis (Macquart) are hematophagous ectoparasites that infest different animal species and occasionally bite humans. Hosts are located by a complex process involving different kinds of stimuli perceived mainly by specific sensory structures on the antennae, which are the essential olfactory organs. General antennal morphology, together with distribution and ultrastructure of sensilla, have been studied in detail with scanning and transmission electron microscopy approaches. Observations have revealed some common features among the four studied hippoboscids: (a) typical concealment of the flagellum inside the other two segments; (b) characteristic trabecular surface of the flagellum; (c) peculiar external microtrichia; (d) presence on the flagellum of basiconic sensilla and grooved peg coeloconic sensilla; (e) unarticulated arista. The ultrastructure of L. fortisetosa revealed that microtrichia and the flagellar reticulated cuticle are not innervated. Different roles have been hypothesized for the described antennal structures. Microtrichia and the reticulated cuticle could convey volatile compounds towards the flagellar sensory area. Peculiar sensory neurons characterize the unarticulated arista which could be able to detect temperature variations. Coeloconic sensilla could be involved in thermoreception, hygroreception, and carbon dioxide reception at long distances, while the poorly porous basiconic sensilla could play a role in the host odour perception at medium–short distances.