Antenna and reaction-center processes upon picosecond-flash excitation of membranes of the green photosynthetic bacterium Chloroflexus aurantiacus

Abstract

The formation and decay of antenna-excited states and the primary charge separation in membranes of the green photosynthetic bacterium Chloroflexus aurantiacus were studied by means of picosecond absorbance difference spectroscopy. After chemical oxidation of the primary electron donor, a 35 ps excitation pulse at 532 nm produced singlet- and triplet-excited states of carotenoid and of bacteriochlorophyll a. Excitation of bacteriochlorophyll a caused a bleaching of its Q y absorption band and induced a blue shift of several neighboring bacteriochlorophyll molecules. The singlet-excited state decayed biphasically with lifetimes of about 200 ps and 1.2 ns. A decrease in the lifetime at increasing flash intensity was attributed to singlet-singlet annihilation. In the presence of active reaction centers also the primary-charge separation and secondary electron transfer were observed. The charge separation consisted of the transfer of an electron from the primary donor, P-865, to the primary-acceptor complex of bacteriopheophytin a and bacteriochlorophyll a. Electron transfer to a secondary acceptor occurred with a time constant of 400 ± 50 ps, which is about 30% longer than had been observed with isolated reaction centers (Kirmaier, C., Holten, D., Mancino, L.J. and Blankenship, R.E. (1984) Biochim. Biophys. Acta 765, 138–146). When this secondary acceptor was prereduced chemically, the lifetime of the primary radical pair increased to 10 ns or more.