Abstract
BackgroundThe native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops.ResultThe objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation.ConclusionsWe concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg.
Highlights
The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops
We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg
Characterization of endophytic actinobacteria Ten isolates of putative endophyte actinobacteria were obtained from native Chiloé potatoes differentiated by their phenotypic characterization and 16S ribosomal gene analysis (Table 1, Fig. 3)
Summary
The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Carotovorum and Pectobacterium atrosepticum are the etiological agents that cause blackleg [5] and soft rot (tubers) diseases on potato crops [6, 7]. They may enter the host through natural apertures (lenticels) or wounds and colonize the plant tissue without causing apparent signs or symptoms of the disease until the environmental conditions (free water, anaerobiosis, and temperature) are propitious for the disease development [4, 8]. The virulence of Pectobacterium sp. relies on plant cell wall-degrading enzymes (PCWDE), which disrupt the host cell integrity; they activate the synthesis of theses exoenzymes when reaching a high population density coordinated by quorum sensing [9] through a complex set of transcription factors and posttranscriptional regulators [10]
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