ANTAGONISTIC ACTIVITY AND PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM FOREST PLANT RHIZOSPHERE AGAINST FUSARIUM SOLANI ON THUJA SEEDLINGS

B Yasin,O Omer Ali,T Sulaiman Rashid

doi:10.36103/ijas.v52i6.1492

Abstract

Root diseases are one of the main forest nursery problems that have a significant impact on forest production which are caused by Fusarium solani. Rhizobacteria from healthy forest soils were isolated and screened in streak method to select antagonistic strains against F. solani. Two isolates showed high antagonistic activity and molecularly identified as Paenibacillus sp. and Pseudomonas sp. The capability of the Paenibacillus sp. and Pseudomonas sp. were tested in greenhouse plastic containers experiments against F. solani. Soil bacterization with Paenibacillus sp. and Pseudomonas sp. significantly protected thuja seedlings from F. solani compared to the untreated control seedlings. The containers added by Paenibacillus sp. and pseudomonas sp. are also showed plant growth promotion including shoot length, root length, dry and wet weights of the seedlings as well as the chlorophyll contents of the thuja seedlings compared to the untreated control plants. In this research it has been showing that the rhizobacterial treatments have potential to decrease the effect of fungal disease severity, promoting the plant growth and also helps plants to maintain a good health.

Highlights

Thuja sp. is a coniferous small genus that has many existing species, including Thuja occidentalis which is known as white ceda
An interest biological control has increased which concerns over the use of chemicals by using beneficial microorganisms including some soil bacterial strains that have many beneficial effects on plant health [14]
Many Plant bacterial interactions are determining in the rhizosphere within the plant health and soil fertility. Some beneficial bacteria such as Bacillus sp. and Pseudomonas sp. produce different biological active compounds and are isolated and cultured in laboratory, it has received a great attention as a biological control agents which can control different disease among them fungal pathogens inside the greenhouse and in the field [3, 19]

Summary

Introduction

Thuja sp. is a coniferous small genus that has many existing species, including Thuja occidentalis which is known as white ceda. To elucidate the role of application of bacterial isolates on disease severity in thuja plants treated with bacteria (the most promising rates based on the results of in vitro experiments), evaluation was done by visually assessing the lesions caused by F.

Results

Conclusion