Abstract
Background Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, SCN5A, Keap1, Nrf2, and HO-1 gene in H9c2 cells, which further illustrate the mechanism. Conclusion ABLP provided protective and therapeutic benefits against H2O2-induced H9c2 cell injury, indicating that this formula can effectively treat coronary disease. In addition, the present study also provides an in-depth understanding of the pharmacological functions of ABLP, which may lead to further successful applications of Mongolian medicine.
Highlights
Cardiovascular disease (CVD) is a leading cause of death globally, contributing to more than 17 million deaths in 2017, of which mortality of coronary heart disease (CHD) is the most prevalent
According to the original composition of this formula, 4 fundamental components were detected according to 2020 China Pharmacopoeia by an UltiMate 3000 high-performance liquid chromatography (HPLC) system. e major compounds in ABLP were detected using the high-performance liquid chromatography (HPLC) method according to the Chinese Pharmacopoeia (2020 edition), and the contents of compounds in ABLP were about 0.34 mg/g dehydrodiisoeugenol, 1.85 mg/g costunolide, 1.88 mg/g dehydrocostus lactone, and 0.64 mg/g gallic acid, respectively
Methylthiazoltetrazolium bromide (MTT) assay and real-time cell analyzer (RTCA) showed that 0.5–50 μg/mL ABLP could significantly increase H2O2-induced cell injury (Figures 1(b) and 1(c))
Summary
Cardiovascular disease (CVD) is a leading cause of death globally, contributing to more than 17 million deaths in 2017, of which mortality of coronary heart disease (CHD) is the most prevalent. TMM medical records consider that ABLP has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath [4]. E results show that compared with compound Danshen tablets, ABLP is better in clinical efficacy and ECG results, and ABLP is more effective in the treatment of angina pectoris. Anlusi [6] randomly divided 112 patients with angina pectoris of coronary heart disease into the treatment group (n 56) and control group (n 56). E control group was treated with metoprolol succinate sustained release tablets, and the treatment group was treated with ABLP for 4 weeks. E combination of ABLP and metoprolol succinate sustained release tablets can significantly reduce the frequency and duration of angina pectoris, effectively improve the symptoms of patients, and improve the quality of life. The pharmacological effect and mechanism need further clarification. erefore, an ameliorative effect of ABLP on cardiomyocytes injury should be further investigated to improve the development and utilization of the formula. e present study was conducted to evaluate the influence of ABLP on H2O2-induced H9c2 cardiomyocytes injury. e cell apoptosis and viability, intercellular Ca2+, and oxidative stress indices, as well as mitochondria function, were detected to discuss the action pathways
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