Abstract
Three new 21-membered macrocyclic benzenoid ansamycins, trienomycins J-L (1-3), together with seven known analogues, trienomycins A-G (4-10), were isolated from liquid culture of the moss soil-derived actinomycete Streptomyces cacaoi subsp. asoensis H2S5. The structures of the new compounds were elucidated by extensive NMR spectroscopic analysis and HRESIMS data. The absolute configurations of trienomycins were established by Marfey's method. Antiproliferative assays showed that compound 1 had the greatest activity against HepG2 cells, with an IC50 value of 0.1 μM. The induction of apoptosis of HepG2 cells by 1 was investigated by flow cytometry and evaluation of nuclear morphology. In addition, all of the compounds inhibited nitric oxide production with IC50 values of 0.02 to 8.3 μM, and compounds 1, 4, and 7 were the most potent inhibitors. These findings will facilitate the development of new antineuroinflammatory agents.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
