Abstract
Summary When mitochondrial particles are energized by addition of substrate and O2 in presence of ANS and a saturation level of Mg2+ (3.3 mM), a change in fluorescence intensity is consistently observed first in presence and then in absence of O2. These changes have been correlated with the properties of ANS as both a cationic ionophore and uncoupler—properties hitherto unrecognized. Divalent metal (Mg2+ or Ca2+) is absolutely required for these changes in fluorescence intensity induced by energization. The causal sequence of events appears to be that energization leads to active transport and redistribution of the Mg2+ complex of ANS in the inner membrane, and this change in localization inevitably leads to a change in fluorescence intensity. Thus it is the cation transport capability of ANS that underlies the changes in fluorescence induced by energization of mitochondrial particles.
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