Anreicherung und eigenschaften von alkalischer phosphatase aus erythrocyten bei familiärer erythrocytose

Abstract

Alkaline phosphatase activity in red cells was detected in members of a family, showing signs of erythrocytosis. A marked purification of the enzyme could be achieved applying the same methods, which are commonly used for the purification of other alkaline phosphatases. The red cell alkaline phosphatase in comparison with other phosphatases shows a similar pH optimum and K m value, on the other hand, it appears to be a new type of enzyme in view of the finding that it exhibits different responses to heat inactivation, phenylalanine inhibition, and immunoelectrophoresis.