Abstract
A new thermophilic spore-forming strain MR3C T was isolated from geothermal soil located on Mount Rittmann in Antarctica. Strain MR3C T was Gram-positive, rod-shaped, occurring in pairs or filamentous. Growth was observed between 45 and 65 °C (optimum 61 °C) and at pH 5.0–6.5 (optimum pH 5.6). It was capable of utilizing galactose, trehalose, maltose and sucrose. The microorganism produced an exopolysaccharide and synthesized an extracellular constitutive amylolytic activity. The G+C content of DNA was 43.5 mol%. On the basis of 16S rRNA gene sequence similarity, strain MR3C T was shown to be related most closely to Anoxybacillus species. Chemotaxonomic data (major isoprenoid quinone–menaquinone-7; major fatty acid– iso-C15:0 and iso-C17:0) supported the affiliation of strain MR3C T to the genus Anoxybacillus. The results of DNA–DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MR3C T from the validly published Anoxybacillus species. MR3C T therefore represents a new species, for which the name Anoxybacillus amylolyticus sp. nov., is proposed, with the type strain MR3C T (=ATCC BAA-872 T=DSM 15939 T=CIP 108338 T).
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