Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system.

Bin Lai,Shiqin Yu,Korneel Rabaey,Jens O Krömer,Paul V Bernhardt,Bernardino Virdis

doi:10.1186/s13068-016-0452-y

Bin Lai, Shiqin Yu + Show 4 more

Open Access

https://doi.org/10.1186/s13068-016-0452-y

Copy DOI

Abstract

BackgroundPseudomonas putida is a promising host for the bioproduction of chemicals, but its industrial applications are significantly limited by its obligate aerobic character. The aim of this paper is to empower the anoxic metabolism of wild-type Pseudomonas putida to enable bioproduction anaerobically, with the redox power from a bioelectrochemical system (BES).ResultsThe obligate aerobe Pseudomonas putida F1 was able to survive and produce almost exclusively 2–Keto-gluconate from glucose under anoxic conditions due to redox balancing with electron mediators in a BES. 2-Keto-gluconate, a precursor for industrial anti-oxidant production, was produced at an overall carbon yield of over 90 % based on glucose. Seven different mediator compounds were tested, and only those with redox potential above 0.207 V (vs standard hydrogen electrode) showed interaction with the cells. The productivity increased with the increasing redox potential of the mediator, indicating this was a key factor affecting the anoxic production process. P. putida cells survived under anaerobic conditions, and limited biofilm formation could be observed on the anode’s surface. Analysis of the intracellular pools of ATP, ADP and AMP showed that cells had an increased adenylate energy charge suggesting that cells were able to generate energy using the anode as terminal electron acceptor. The analysis of NAD(H) and NADP(H) showed that in the presence of specific extracellular electron acceptors, the NADP(H) pool was more oxidised, while the NAD(H) pool was unchanged. This implies a growth limitation under anaerobic conditions due to a shortage of NADPH and provides a way to limit biomass formation, while allowing cell maintenance and catalysis at high purity and yield.ConclusionsFor the first time, this study proved the principle that a BES-driven bioconversion of glucose can be achieved for a wild-type obligate aerobe. This non-growth bioconversion was in high yields, high purity and also could deliver the necessary metabolic energy for cell maintenance. By combining this approach with metabolic engineering strategies, this could prove to be a powerful new way to produce bio-chemicals and fuels from renewables in both high yield and high purity.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0452-y) contains supplementary material, which is available to authorized users.

Highlights

Pseudomonas putida is a promising host for the bioproduction of chemicals, but its industrial applications are significantly limited by its obligate aerobic character
This electron flux is indicative of the anoxic catabolism of glucose since this was the only electron donor in the system at sufficiently high concentration to induce a transfer of charge of over 850 °C in 218 h
The concentration of planktonic cells decreased over time similar to the controls lacking the anode (Fig. 1a), but a non-homogeneous biofilm formation on the carbon cloth electrode could be observed during the electrochemical experiments (Additional file 1: Fig. S6)

Summary

Introduction

Pseudomonas putida is a promising host for the bioproduction of chemicals, but its industrial applications are significantly limited by its obligate aerobic character. A range of products are currently produced in biotechnological processes [3], including enzymes, amino acids, antibiotics, alcohols, organic acids and vitamins using ever-expanding range of evolved and genetically engineered microorganisms Such biotechnological processes, often face limitations based on redox balance, carbon yields or product toxicity. A class of microbes that was recently recognised as a promising new platform for the production of chemical feedstocks (often toxic even to the microbial production strain) are the pseudomonads [4] They have been used to produce antimicrobial aromatics such as phenol [5] and show, in comparison with other industrial organisms such as Escherichia coli or baker’s yeast, particular advantages in solvent tolerance [6, 7]. A range of aromatic compounds, such as 3-methylcatechol [12] and p-hydroxybenzoic acid [13], have been produced with P. putida

Objectives

Methods

Results

Conclusion