Abstract
Jojoba [Simmondsia chinensis (Link) Schneider] plant has immense industrial value. Jojoba is grown for its seed oil, which is a unique liquid wax consisting of esters formed from acids and alcohols with chain lengths of 20 or 22 carbon atoms. Higher seed yield is obtained when well balanced male and female plants are established in jojoba orchard. However, the sex of jojoba plants cannot be determined with morphological characters until the plants reach reproductive maturity. DNA markers are commonly used for plant genetic studies [1,2,3,4], Up-to-date several male and female specific DNA markers have been developed for sex identification in jojoba seedlings [5,6]. This study reports another cleavage-amplified polymorphic sequence (CAPS) assay, which easily identify male individuals. Genomic DNAs of 16 male and 16 female jojoba plants growing in an orchard were extracted using a DNA extraction protocol [4]. A touch-down PCR approach was used to amplify genomic DNAs of jojoba using J818F 5'-AGGGGATAAATGAGCCGAAT-3' and J818R 5'-GACCCAGAGGATGAGGAATG-3' primer pair [6]. Amplified products were digested using Hind III restriction enzyme. Electrophoresis separation of the digested products as shown in Figure 1 indicated that male and female jojoba plant can be easily identified. CAPS marker reported in this study could be used in breeding studies and in the sex allocation of seedlings in seed orchard establishment.
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