Anopheles culicifacies breeding in brackish waters in Sri Lanka and implications for malaria control

Pavilupillai J Jude,Ranjan Ramasamy,Muthuladchumy Vinobaba,Sinnathamby N Surendran,Sangaralingam Dharshini

doi:10.1186/1475-2875-9-106

Abstract

BackgroundAnopheles culicifacies is the major vector of both falciparum and vivax malaria in Sri Lanka, while Anopheles subpictus and certain other species function as secondary vectors. In Sri Lanka, An. culicifacies is present as a species complex consisting of species B and E, while An. subpictus exists as a complex of species A-D. The freshwater breeding habit of An. culicifacies is well established. In order to further characterize the breeding sites of the major malaria vectors in Sri Lanka, a limited larval survey was carried out at a site in the Eastern province that was affected by the 2004 Asian tsunami.MethodsAnopheline larvae were collected fortnightly for six months from a brackish water body near Batticaloa town using dippers. Collected larvae were reared in the laboratory and the emerged adults were identified using standard keys. Sibling species status was established based on Y-chromosome morphology for An. culicifacies larvae and morphometric characteristics for An. subpictus larvae and adults. Salinity, dissolved oxygen and pH were determined at the larval collection site.ResultsDuring a six month study covering dry and wet seasons, a total of 935 anopheline larvae were collected from this site that had salinity levels up to 4 parts per thousand at different times. Among the emerged adult mosquitoes, 661 were identified as An. culicifacies s.l. and 58 as An. subpictus s.l. Metaphase karyotyping of male larvae showed the presence of species E of the Culicifacies complex, and adult morphometric analysis the presence of species B of the Subpictus complex. Both species were able to breed in water with salinity levels up to 4 ppt.ConclusionsThe study demonstrates the ability of An. culicifacies species E, the major vector of falciparum and vivax malaria in Sri Lanka, to oviposit and breed in brackish water. The sibling species B in the An. subpictus complex, a well-known salt water breeder and a secondary malaria vector in the country, was also detected at the same site. Since global warming and the rise in sea levels will further increase of inland brackish water bodies, the findings have significant implications for the control of malaria in Sri Lanka and elsewhere.

Highlights

Anopheles culicifacies is the major vector of both falciparum and vivax malaria in Sri Lanka, while Anopheles subpictus and certain other species function as secondary vectors
Species B and E in the An. culicifacies complex of A-E have been detected in Sri Lanka with species E being incriminated as the major vector of falciparum and vivax malaria [5,6]
Anopheles culicifacies larvae were collected from freshwater bodies within one km proximity to the Kalladay locality and brackish water bodies in at a different site called Raalkuli in the tsunami-affected Trincomalee district of the Eastern province of Sri Lanka in the period from December 2009 to February 2010

Summary

Introduction

Anopheles culicifacies is the major vector of both falciparum and vivax malaria in Sri Lanka, while Anopheles subpictus and certain other species function as secondary vectors. Anopheles culicifacies sensu lato (s.l.) is well established as the major vector of both falciparum and vivax malaria in Sri Lanka, while Anopheles subpictus s.l. and certain other species function as secondary vectors [1,2,3,4]. Both vector species exist as a species complex in Sri Lanka and elsewhere in Asia [reviewed in [1]]. Batticaloa and other tsunami-affected eastern districts (Trincomalee and Ampara) of Sri Lanka had a high incidence of malaria before the tsunami [10], but the decrease in malaria incidence beginning 2004 showed that the tsunami did not increase malaria transmission in Batticaloa (Table 1) or elsewhere in the country (Figure 1)

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