Abstract
Segregation of the linked group of loci, S, Gal, Ara, Xyl and Mal in crosses of E. coli is such that these can not be arranged in linear sequence by the usual methods. Assuming that there is no great deviation from familiar chromosome behavior, this anomaly appears to be due to an excess of double crossovers too great to be attributable to irregularities in chromosome pairing, and not such as could result from a selective mechanism acting against single crossovers (e.g., an inversion, a ring chromosome, or a combination of lethal genes). This negative interference in crossing-over seems to occur in all chromosome regions tested.
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