Abstract
SUMMARY: Application of the anomalous diffraction theory of van de Hulst to turbidity measurements of Gram-positive bacteria is discussed. It is shown that this method is capable of detecting characteristic morphological changes during growth. Comparison with the cell mass method and direct observations of Micrococcus freudenreichii suggest that these changes are primarily related to cluster size. Initial application of the method in the study of osmotic change in Bacillus megaterium is described.
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