Abstract
SINCE OUR FIRST PUBLICATION (I) ON THE ASSAY of prolactin by means of the weight of the crop-sacs of doves and pigeons we have studied various factors (2) which modify the extent to which a specific quantity of prolactin increases the weight of the cropsacs. The present study was undertaken to determine the re producibility throughout the year of the crop-sac weights obtained at any one time or season. A constant amount of the same preparation of prolactin was used in all tests, and groups of 10 birds were tested at intervals of 4 to 6 weeks during two years. During the first year of study large and unanticipated variations in crop-sac weights were observed at certain periods, and it was therefore decided to continue the study throughout a second year in order to verify the findings of the first year.
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