AnnexinA1 expression is correlated with malignant potential of renal cell carcinoma.

Abstract

To evaluate the expression of annexinA1 protein in patients with renal cell carcinoma. AnnexinA1 expression was examined in renal cell carcinoma specimens from 27 patients, and their disease-free survival was analyzed using the log-rank test. AnnexinA1 knockdown in the human renal cell carcinoma cell line Caki-1 was carried out, and its proliferation, invasion, motility and adhesion were compared with those of control cells. In 13 out of 27 patients, annexin A1 was highly expressed in the membrane of renal cell carcinoma tumor cells, whereas in the rest of the patients, annexinA1 expression was weak or negligible in the membrane of those cells. Patients with high annexinA1 expression had significantly poorer disease-free survival than those with weak or negligible annexinA1 expression (P=0.031). In the renal cell carcinoma cell line, annexinA1 knockdown cells showed significantly decreased proliferation, invasion, motility and adhesion relative to control cells, and expressed lower relative levels of membrane-type1 matrix metalloproteinase and hypoxia-inducible factor1-alpha transcripts, showing a potential pathway regulated by annexinA1. Annexin A1 is associated with renal cell carcinoma malignant potential and could serve as a marker of poor prognosis.