Abstract
The objective is to describe the impact annexin Vbinding technique has on couples diagnosed with male infertility and how to optimize sperm parameters so that this technique contributes to simplify the assisted reproductive treatment that will be prescribed [1-4]. Empirical studies and expert consensus related to the physiological and medical aspects of the use of annexin V-binding which were presented in this study were identified in articles through bibliographical research.
Highlights
The apoptosis or programmed cell death consists of various cellular mechanisms before the end of the process occurs
Apoptotic markers used at present in human spermatozoa include the analysis of phosphatidylserine externalization’s levels or early apoptosis and in the case of late apoptosis, they include active caspase-3 and DNA fragmentation [5]
Numerous publications report that the sperm quality with annexin V-binding or its acronym in English: MACS (Magnetic Activated Cell Sorting), when it is performed after the density gradient centrifugation, results in the separation of a sperm population of the highest quality especially evidenced in its mobility and viability levels as well as in a decreased presence of apoptotic markers [10]
Summary
The apoptosis or programmed cell death consists of various cellular mechanisms before the end of the process occurs. Apoptotic markers used at present in human spermatozoa include the analysis of phosphatidylserine externalization’s levels or early apoptosis and in the case of late apoptosis, they include active caspase-3 and DNA fragmentation [5]. The DNA damage may be simple and/or double-strand breaks and it has been assessed by different methods, among which the TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) technique stands out [6]
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