Annexin V and platelet antigen expression is not altered during storage of platelet concentrates obtained with the AMICUS™ cell separator

Abstract

During storage of platelet concentrate the so-called “storage lesion” occurs. During this time, platelets loose their morphological and functional capacities that are necessary for proper in vivo efficacy following transfusion. Annexin V represents a marker for apoptosis. In this study, Annexin V and additional antigens were analyzed by flow cytometry. Platelet concentrates were obtained with a new cell separator (AMICUSTM Separator, Fenwal). Following apheresis, platelet units were stored for an experimentally prolonged time of seven days. Daily aliquots of the platelet-rich plasma were obtained to measure Annexin V and platelet antigens CD62p, CD63, CD41a, CD42b, and the binding of fibrinogen. All analyses were performed using flow cytometry. During storage, no significant changes in mean channel fluorescence intensity (MCFI) of CD41a (P=0.99) and CD42b (P=0.29), percentage of CD62p+ and CD63+ platelets (P=0.23 for CD62p; P=0.52 for CD63), and the binding of fibrinogen to platelets occured (P=0.85). Also, the expression of Annexin V remained constant with no significant change (P=0.36). This study shows that antigens of platelets, obtained with the AMICUSTM cell separator are well preserved during storage. Regarding Annexin V, no obvious signs of apoptosis can be detected by flow cytometry. These findings demonstrate the high degree of biocompatibility of the apheresis device and storage container.