Abstract
Annexin-I is a calcium dependent phospholipid binding and phospholipase A 2 (PLA 2) inhibitory protein. A ‘substrate depletion’ model has been proposed for the mechanism of PLA 2 inhibition by annexin-I in studies with 14 to 18 kDa PLA 2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA 2 from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA 2 inhibition by annexin-I was consistent with a ‘specific interaction’ mechanism rather than the ‘substrate depletion’ model. Apparent contradiction with previous studies can be explained by the calcium-dependent binding of annexin-I to the substrate.
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